Approximately 1,700 songs were allegedly subject to copyright violations, according to a lawsuit filed by 17 music publishers in the US against Twitter.
More than $250 million (£197.7 million) in damages are sought by the National Music Publishers' Association (NMPA).
The NMPA filed a lawsuit in Federal District Court in Nashville alleging that Twitter "permits and encourages infringement" for financial gain.
It claims that since Elon Musk purchased the business, things have not gotten better.
The National Music Publishers Association (NMPA), which represents companies like Sony Music Publishing, BMG Rights Management, and Universal Music Publishing Group, claimed that Twitter continues to "reap huge profits from the availability of unlicensed music without paying the necessary licensing fees for it.".
The company also claimed that Twitter now has an "unfair advantage" over rivals who purchase music licenses, such as TikTok, Facebook, Instagram, YouTube, and Snapchat.
The NMPA's David Israelite said in a statement that Twitter "stands alone as the largest social media platform that has completely refused to license the millions of songs on its service.".
An inquiry from the BBC for comment received no direct response from Twitter.
Mr. Musk, who recently reclaimed the title of world's richest person, paid $44 billion to acquire Twitter last year.
The NMPA added, "Twitter has not changed its behavior with regard to copyright since its ownership changed in October 2022. ".
The statement continued, "On the contrary, Twitter's internal affairs regarding matters pertinent to this case are in disarray.
The NMPA cited Twitter's elimination of "critical departments involved with content review and policing terms of service violations" as well as the resignations of Yoel Roth and Ella Irwin, the company's trust and safety chiefs.
Additionally, according to the NMPA, Twitter "regularly ignores known repeat infringers and known infringements.".
Linda Yaccarino, the former head of advertising at media behemoth NBCUniversal, was appointed to lead the troubled social media company earlier this month.
The platform, which has had trouble turning a profit, is managed by Ms. Yaccarino.
Since acquiring Twitter, Mr. Musk has changed how the company verifies accounts and cut 75% of its staff, including teams responsible for monitoring abuse.
