According to organizers, more than 1,000 Amazon employees took part in a one-hour protest against the internet company's track record on climate change and its push to send office workers back to their desks.
Staff advocacy organizations organized the protest.
They claimed that a string of "short-sighted decisions" by the company's leaders had caused morale to reach an "all-time low.".
Amazon denied there were any participants, but added that it respected the right of its employees to express their opinions.
At the company's Seattle headquarters, it claimed that its security team estimated that more than 300 employees were taking part in the protest, and it was not aware of any additional physical walkouts.
About 2,000 staff members had committed to participate before the event, including about 900 in Seattle, according to the organizers.
"Our goal is to change Amazon's cost-benefit analysis on making harmful, unilateral decisions that are having an outsized impact on people of color, women, LGBTQ people, people with disabilities, and other vulnerable people," according to the groups Amazon Employees for Climate Justice and Amazon's Remote Advocacy group.
The protest comes as Amazon has been trying to cut costs, partly in response to the uncertain economy that has slowed sales in many areas of the business, including e-commerce and its cloud computing division.
Since January, it has disclosed 27,000 layoffs and reduced spending in numerous areas, including halting construction at a prominent corporate campus close to Washington, DC.
The company, which employs more than 350,000 corporate and tech workers worldwide and more than 65,000 of them close to its headquarters, also mandated that office staff spend at least three days a week in the office, beginning in May. The company claimed that this would enhance communication within the company and the culture.
In reference to a company catchphrase created by Amazon founder Jeff Bezos, who called for "day 1" thinking focused on innovation and long-term goals, organizers claimed that leaders were engaging in "day 2 decision-making and taking us in the wrong direction.".
They stated that they wanted the company to prioritize the environment in its decision-making and more flexibility in their working arrangements.
They also charged the business with understating its carbon footprint, citing a report that claimed Amazon only took Amazon-brand goods into account when making its calculations.
Despite being "always listening" and "continuing to do so," company spokesman Brad Glasser said, "we're pleased with how the first month of having more people back in the office has gone.".
He claimed that Amazon was on track to completely rely on renewable energy sources by 2025 and was still committed to keeping its 2019 promise to be carbon neutral by 2040.
Among other actions, the company has electrified its delivery fleet.
To achieve our goal, he declared, "We will keep making sizable investments, coming up with new ideas, and working with people both internally and externally."