Pep Guardiola, Manchester City's manager, claims that the team has "already been condemned" for alleged financial rule violations.
On Monday, the Premier League accused City of breaking more than 100 of its financial regulations.
The Court of Arbitration for Sport (CAS) overturned a two-year ban from participating in European competitions in 2020 due to a violation of Uefa's financial fair play rules.
Guardiola claimed that the club had "completely established" their innocence.
"What has occurred since Monday is identical to what occurred with Uefa. We have already been declared guilty.
"You have to realize that we are being accused by 19 Premier League teams without having a chance to respond.
"I have no doubt that we will not be guilty.
The Premier League referred City to an impartial commission due to alleged rule violations that occurred between 2009 and 2018, when the club won three of its six Premier League championships.
Additionally, it claimed that City had refused to assist ever since the investigation began in December 2018.
The commission has the authority to impose penalties, which can include expulsion from the Premier League as well as fines and point deductions.
City, who were acquired by the Abu Dhabi United Group in 2008, stated that they were "surprised" by the allegations and that there is "a body of irrefutable evidence" to back them up.
In a defiant news conference on Friday, Guardiola reaffirmed that confidence and added that he thought the accusations were the work of rival clubs.
If he thought that was the case, he responded, "Of course, it's the Premier League. I'm not sure why. The CEOs must be questioned.
With what they did to us, they have already established a precedent.
"Be careful, be careful in the future. There are many clubs that have been accused, as we have been, without being found not guilty. Who knows what will happen in the future?
"They think we acted improperly; we can accept that, but let's defend when we think we acted appropriately.
There will be more.
